A preparative high performance liquid chromatography method for separation of lecithin: comparison to thin-layer chromatography

A preparative high performance liquid chromatography method for separation of lecithin: comparison to thin-layer chromatography.

We have developed a high performance liquid chromatography (HPLC) method to separate lecithin from other phospholipid classes and to obtain lecithin from biologic materials. The separation was performed on a preparative 10-micron Spherisorb column with an optimized solvent system consisting of the following components: acetonitrile, isopropanol, methanol, water, and trifluoroacetic acid. The ad...

Preparative Thin-Layer (Planar) Chromatography

The various OPLC andRPC techniques are compared in Table 1. Study of the data shows that OPLC is an excellent technique for analytical separations and that RPC is more ideally suited as a preparative method for isolation of compounds from biological matrices. The advantage of combining online and ofSine separations and two-dimensional development can also be exploited in OPLC. The advantage of ...

High-performance Liquid Chromatography Separation

A h igh-per formance l i q u i d chromatography (HPLC) method has been deve loped f o r t h e s e p a r a t i o n of o l e f i n , s a t u r a t e , and a r o m a t i c hydrocarbons found i n h i g h b o i l i n g d i s t i l l a t e s and r e s i d u e s of s h a l e o i l and i n whole s h a l e o i l s . The dual-column chromatographic system uses s i l i c a g e l i n one column and s i l i...

A Novel High-Performance Liquid Chromatography Method for Detection of Alginate in Pseudomonas aeruginosa

Background and Objective: The opportunistic pathogen Pseudomonas aeruginosa secrets a capsule-like polysaccharide called alginate which is important for evasion of host defenses, especially in patients with suppressed immunity. Method of alginate determination has an important role in the study of microb...

a high-performance thin-layer chromatography method for quantitative determination of quercetin in herbal products